Cattle OCT(Ornithine Carbamoyl Transferase) ELISA Kit

General information

Catalog number

ELK7939

Size

96T

Price

738.00 €

Detailed information

Alternative Names

OCTD; OTCase; Ornithine Transcarbamylase; Ornithine Carbamoyltransferase

Assay Type

Sandwich

Sensitivity

2.49ng/mL

Standard

400ng/mL

Detection range

6.25-400ng/mL

Assay length

3h

Research Area

Enzyme & Kinase;Metabolic pathway;

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Carbamoyl Transferase (OCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Carbamoyl Transferase (OCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Carbamoyl Transferase (OCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ornithine Carbamoyl Transferase (OCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED