General information
Catalog number
ELK7939
Size
96T
Price
738.00 €
Detailed information
Alternative Names
OCTD; OTCase; Ornithine Transcarbamylase; Ornithine Carbamoyltransferase
Assay Type
Sandwich
Sensitivity
2.49ng/mL
Standard
400ng/mL
Detection range
6.25-400ng/mL
Assay length
3h
Research Area
Enzyme & Kinase;Metabolic pathway;
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Carbamoyl Transferase (OCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Carbamoyl Transferase (OCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Carbamoyl Transferase (OCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ornithine Carbamoyl Transferase (OCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED